The DOSY sequence includes a delay (for diffusion) and a pulse (the gradient pulse). It is a necessity to know their values, otherwise the raw data can only be processed as an array of 1-D spectra. The exponential decay (due to diffusion) of the signals can be expressed in the general form:

`signal(z) = max(signal) exp(-zD)`

where D is the diffusion constant and z a function of the diffusion delay and the gradient pulse.
A script does the job of extracting the values from the original files and calculating
the list of z values.
iNMR itself only reads the calculated values of z from a text file called
“zeta”, already written by the script.
Examples of scripts can be found on the web site.
You need to run the script only once (to create the file “zeta”), with the DOSY experiment
already open and into the foreground.
You are free to edit this file or generate it with your own program or script, using the language of your choice.

Note that the quantity of zD is dimensionless. You are free and responsible to choose any unit for z.
The diffusion coefficients will be expressed in the inverse unit. iNMR will utterly ignore those units.

A DOSY spectrum must first be processed like an arrayed spectrum (for example, like an experiment of inversion recovery). In other words, you process it as a 2-D spectrum, but applying the FT along the rows only. Then it is necessary to correct the phase accurately. A baseline correction might be useful or deleterious. Try with and without and judge by yourself. iNMR assumes that the chemical shifts are constant during the experiment. If this is not the case, apply binning along the rows only: the spectrum will be less resolved, but the peaks will not wander from row to row.

It is important that iNMR recognizes a DOSY experiment as an arrayd experiment. What this means in detail is that iNMR should understand that one of the indirect dimensions (normally f-1) is not suitable for FT processing. This recognition is normally automatic and happens the first time you open the spectrum with iNMR. The program then stores the information by means of a trick, namely by defining a dummy atomic species, whose symbol is a dot. If iNMR fails to recognize a DOSY experiment as an arrayed experiment, open the dialog Edit > Metadata and modify the atomic symbol manually, substituting a dot for the H (for example). Avoid using the “fake FT” option: it solves the problem only partially, because iNMR can't tell which dimension to apply the DOSY processing along.

The command “Process > DOSY > Mono-Exponential” can effectively process spectra where each column contains a single signal, or where all the signals at any given chemical shift belong to a single component of a mixture. In this case it is enough to fit the experiment with a simple mono-exponential decay. Here is the meaning of the parameters:

**max. log D**- You can restrict the range of the diffusion scale. Quite likely, however, iNMR will further restrict your limits to fit the results. iNMR can't, instead, expand your limits. The diffusion coefficients have negative logarithms, therefore higher values mean smaller (in absolute value) negative logarithms. The higher the diffusion, the smaller the molecule.
**min. log D**- The diffusion scale follows the conventiion of the Cartesian plane and increases from bottom to top. At the bottom there are the slowest (and largest) molecules.
**Restrict the Spectral Width...**- You can optionally process just the part of the spectrum that contains the peaks of your interest.
**Final Number of Rows**- While iNMR is free to restrict the vertical scale to match the diffusion coefficients, the number of rows will be exactly the value you require. Any integer quantity between 16 and 1024 is acceptable, it doesn't have to be a power of 2.
**X-Smoothing**- This OPTIONAL filter is a cosmetic manipulation with which the different results of adjacent columns are averaged. This operation improves the alignment of the peaks and makes them more realistic. Set the parameter to zero to skip this treatment. Set it to higher values to get broader and more regular peaks. Use 0 or very small values if you have already “binned” your spectra.
**Max Width in the diffusion dimension**- The DOSY plot is an artificial representation in which the position of each synthetic peak corresponds to the calculated value of a diffusion constant and the width (along the diffusion dimension) is proportional to the root mean squared error. The area is proportional to the natural height of the peak. Like with normal NMR spectra, the user can change the width of the peaks. Unlike normal NMR spectra, the only possibility that is given in our module is to put an upper limit to the RMSE, therefore to the width of the peaks. If a peak is broader than the user-defined limit, it will be narrowed. This limit is expressed in units of points. If you keep this value constant and, at the same time, increase the number of rows, the peaks will actually become narrower (along the diffusion scale). In other words, what really matters is the ratio (Max Width) / (Final Number of Rows). When there are many deviations from a pure exponential decay this correction improves a lot the readability of the plot. The apparent variance will, however, become an optimistic artifact.
**Acceptable Signal / Noise**- This is a preliminary filter to eliminate not only the weakest peaks but, most importantly, the tails of the strongest peaks. The diffusion coefficents are indeed less accurate if measured from the tails.

If you select again the command Process > DOSY > Mono-Exponential and then close the dialog with the button “Cancel” you'll return to the frequency-domain spectrum before the DOSY-like transformation.

The diffusion scale (vertical scale) is either logarithmic (to be more correct, it corresponds to the negative of the logarithm)
or linear. In the latter case, all the diffusion coefficients are multiplied by 1e10.
They are more readable in this way.
An handy method to calculate the value of the diffusion coefficient is to put an horizontal mark on the peak of interest
(or up to 5 such marks on different peaks) and choose the command Process > DOSY > Diffusion Coefficients...

A dialog appears.
The calculated coefficents will be reported on the right, in their true dimension.
You can store them into the document, by clicking the button
at the bottom.

Once you have created a list of diffusion coefficients with the command above, you can extract the single components with the command “Extract Components”. You still get the same kind of DOSY plot but, this time, if a column contains more than one signal, you will get two different peaks. The plot generated by this command contains much less rows than the mono-exponential DOSY, actually you can't specify the number of rows. There are so few rows that it is necessary to use a stacked plot to display the result.

The dialog shows a few options, at the top, that are identical to the ones seen for the mono-exponential case. The completely new parameter is called “Ignore components under ...%”. The percentage is calculated relative to the highest component of the column. If you discover that the separation is not good, increase this value and repeat the processing. The risk is that a true minor component can disappear. If you already know, however, that the signals of the components of the mixture fall at different frequencies, than it's OK to ignore even components under 99%.

If the list of the diffusion coefficients contains more than 2 values, you can select a subset. This strategy can be useful when only a portion of the spectrum is processed.